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Double staining for oligodendroglia and macrophages

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Fluorescent light microscopy (200 magnification) double labeling with 2,3 cyclic nucleotide 3' phosphodiesterase (CNP, red, oligodendroglial membrane marker) and CD68 (blue-white, macrophage marker) antibodies. (Arrow) Red membrane marking by CNP of a foamy oligodendroglial cell. Phase contrast (not shown) of same histologic section demonstrated a small, round oligodendroglia-type nucleus within the CNP-stained membrane. (Contributed by Dr. Regina Armstrong.)