Absorbance spectrum of a vitamin B6 and carboplatin mixture in an incubation buffer over time

The physico-chemical characterization of the interaction of these chemicals was performed using UV-vis (ultraviolet-visible) spectroscopic techniques in the wavelength range from 190 to 500 nm. UV-vis spectrophotometers use a light source to illuminate a sample with light from across the UV to the visible wavelength range of the electromagnetic spectrum. The UV range normally extends from 100 to 400 nm, with the visible range extending from approximately 400 to 800 nm. In an incubation buffer, the maximum absorbance of vitamin B6 was obtained at 323 nm wavelength. After adding the solution of carboplatin to the solution of vitamin B6 in buffer, the maximum absorbance for the vitamin decreased, reflecting a decrease in vitamin B6 concentration. The decrease of absorbance of pyridoxine hydrochloride is caused by formation of a vitamin B6-carboplatin complex. After 48 hours, the vitamin B6 concentration decreased by 28% relative to the baseline vitamin B6 concentration value. (Source: Szefler B, Czeleń P, Krawczyk P. The affinity of carboplatin to B-vitamins and nucleobases. Int J Mol Sci 2021;22[7]:3634. Creative Commons Attribution License [CC BY], creativecommons.org/licenses/by/4.0.)